Total RNA was prepared from cell line WM873-1. WM873-1 is a metastatic human melanoma cell line. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses PTEN loss of function including hemizygous deletion, and is wild type for N-RAS, c-KIT, and CDK4 genes. WM873-1 cells match with WM858 and WM873-2. These cells produce xenograft tumors when injected into immunocompromised mice.
Concentration:
200 ng / µl (by UV absorbance at 260 nm)
Buffer:
RNase free water
Source:
Human
Form:
Liquid
Application Notes:
Purified RNA is suitable for a number of molecular biology applications including but not limited to next generation sequencing and expression library construction, PCR, real-time PCR, microarray analysis, and transfection experiments.
Total RNA from melanoma tumor cells for assessment of mRNA and miRNA
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