SARS-CoV / SARS-CoV-2 Spike Antibody, Rabbit MAb

Catalog Number: SIN-40150-R007
Article Name: SARS-CoV / SARS-CoV-2 Spike Antibody, Rabbit MAb
Biozol Catalog Number: SIN-40150-R007
Supplier Catalog Number: 40150-R007
Alternative Catalog Number: SIN-40150-R007-50,SIN-40150-R007-100
Manufacturer: Sino Biological
Host: Rabbit
Category: Antikörper
Application: ELISA, FC, ICC, IF, IHC-P, IP
Species Reactivity: Virus
Alternative Names: SARS-COV, COVID-19, COVID19, CORONA, COV2, Spike, NCOV, SARS-COV-2, COVID, COV-2

Product name

SARS-CoV-2 (2019-nCoV) Spike S1 Antibody, Rabbit MAb

Validated applications

ELISA

Application notes

IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.)

Specificity

SARS Coronavirus spike
No cross-reactivity in ELISA with
MERS-CoV Spike S1 Protein (Cat# 40069-V08B1)
HCoV-HKU1 (isolate N1) Spike S1 Protein (Cat# 40021-V08H)
HCoV-HKU1 (isolate N5) Spike S1 Protein (Cat# 40602-V08H)
HCoV-NL63 Spike S1 Protein (Cat# 40600-V08H)
HCoV-229E Spike S1 Protein (Cat# 40601-V08H)
HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B)

Preparation

This product is a recombinant monoclonal antibody expressed from HEK293 cells.

Source

Monoclonal Rabbit IgG Clone #007

Purification

Protein A

Formulation

0.2 μm filtered solution in PBS

Conjugate

Unconjugated

Form

Liquid

Shipping

This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

 

Clonality: Monoclonal
Clone Designation: 007
Isotype: IgG
Buffer: 0.2 μm filtered solution in PBS
Target: Spike
Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in ACE2-overexpressed 293T cells, infected (left) or noninfected (right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked with 10% serum, and incubated with SARS-CoV-2 (2019-nCoV) Spike rabbit monoclonal antibody (dilution ratio 1:60) at 37℃ for 1 hour. Then cells were stained with the Alexa Fluor®-conjugated Goat Anti-rabbit IgG secondary antibody (green).