Aconitase (ACO) is an enzyme in the tricarboxylic acid cycle that catalyzes the conversion of citric acid to isocitric acid. Citric acid itself is not easily oxidized. Under the action of aconitase, through dehydration and hydration reactions, the hydroxyl group is transferred from the beta-carbon atom to the alpha-carbon atom, generating isocitric acid which is easy to be dehydrogenated and oxidized, preparing for further oxidative decarboxylation reactions.
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