Tubulin polymerization-IN-84 can inhibit the polymerization process of tubulin (Tubulin) by targeting the colchicine-binding pocket, with an IC50 value of 10.9 µM. Tubulin polymerization-IN-84 exhibits antiproliferative activity against four cell lines, namely Jurkat, B16-F10, HCT116 and MDA-MB-231, with corresponding IC50 values of 60 nM, 380 nM, 138 nM and 1.054 µM, respectively. In B16-F10 cells, Tubulin polymerization-IN-84 can induce G2/M phase cell cycle arrest and further trigger cell apoptosis. In addition, in the B16-F10 melanoma model, it can effectively inhibit the growth of tumor tissue, when combined with PD-L1 monoclonal antibody, it can also enhance the in vivo anti-tumor immune response, and can be used in the related research fields of T-cell acute lymphoblastic leukemia, melanoma, colon cancer and breast cancer.
