Thioester analogs of glycerophospholipids, in combination with Ellmans reagent, are convenient colorimetric substrates for the measurement of phospholipase (PL) activity. Palmitoyl thio-PC is a chromogenic PLA2 substrate that contains a palmitoyl thioester at the sn-2 position of the glycerol backbone. Hydrolysis of the thioester by PLA2 yields a free thiol that reacts readily with DTNB (Ellmans reagent) giving a bright yellow product with an absorbance maximum at 412 nm. Palmitoyl thio-PC has been used to measure bee venom sPLA2 activity in a phospholipid:Triton X-100 mixed micelle system.
Molecular Weight:
750.11
CAS Number:
[113881-60-0]
Formula:
C40H80NO7PS
Target:
Others
T37244
* VAT and and shipping costs not included. Errors and price changes excepted