DPNB-ABT 594 is a nitrobenzyl-caged ABT 594, a selective alpha4beta2 nAChR agonist. One-photon uncaging evokes large inward currents and Ca2+transients on cell bodies and dendrites of medial habenular neurons in mouse brain slices. Two-photon uncaging induces fast nAChR-mediated currents. Photolyzed with high quantum yield of 0.20. Effective photolysis occurs using one- or two-photon excitation, one-photon uncaging requires illumination at 410 nm for 1.5-3 ms, two-photon uncaging requires illumination with appropriate two photon pulse laser at 710 nm for ~2 ms.
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