Verrucarin J (Muconomycin B), derived from the Myrothecium fungus family, effectively generates reactive oxygen species (ROS) to induce apoptosis in various cancer cell lines, including A549, HCT 116, and SW-620. Besides its anticancer properties, it exhibits antifungal activity against Candida albicans and Mucor miehei. Furthermore, it notably inhibits the arenavirus Junin (JUNV) yield with an IC 50 of 1.2 ng/mL.