Rhodamine B hydrazide serves as an effective probe for sulfite detection, transitioning from colorless and non-fluorescent to emitting Rhodamine B-like fluorescence upon interaction with sulfite (5-800 ng/mL, detection limit=1.4 ng/mL (3sigma)). This process occurs as sulfite reduces dissolved oxygen, generating superoxide radicals that react with Rhodamine B hydrazide to yield Rhodamine B. The addition of Tween 80 surfactant micelles further enhances this fluorescence. The absorption and fluorescence emission maxima of Rhodamine B hydrazide are at 554 nm and 574 nm, respectively [1].
Molecular Weight:
456.58
CAS Number:
[74317-53-6]
Formula:
C28H32N4O2
Target:
Others
T81276
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