Sodium Citrate Buffer (0.5M, pH 6.5) is a sodium citrate buffer with a pH of 6.5. It is a commonly used acidic biochemical buffer suitable for heat-induced epitope retrieval (HIER) to reduce antigen masking caused by protein cross-linking in formalin-fixed, paraffin-embedded (FFPE) tissues. Sodium Citrate Buffer (0.5M, pH 6.5) also maintains stable pH within its effective buffering range, making it suitable for stabilizing certain proteins and enzymes.
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