A single biotin moiety is conjugated on the N-terminal region of ubiquitin. All lysine residues of ubiquitin are still available for formation of polyubiquitin chains. The extremely high binding affinity between biotin and streptavidin allows the use of biotin-ubiquitin for the following in vitro assays: 1) rapid and efficient purification of polyubiquitinated substrate proteins, 2) monitoring substrate ubiquitination using an HRP-conjugated streptavidin antibody, and 3) setting up high throughput FRET assays to monitor substrate or ubiquitin ligase ubiquitination.
Molecular Weight:
8.9 kDa
Buffer:
20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Source:
chemical synthesis
* VAT and and shipping costs not included. Errors and price changes excepted
