TAMRA-Ubiquitin is a fluorescent ubiquitin, in which TAMRA (5-tetramethylrhodamine) is covalently conjugated onto the N-terminal region of human ubiquitin. All seven lysine residues and the N-terminus methionine of ubiquitin are available for ubiquitination. This product can be used for determination of protein ubiquitination or E1/E2/E3 enzyme activity using sensitive in-gel TAMRA fluorescence that can be detected using excitation/emission wavelengths at 550 nm/590 nm, respectively. Typically, 20 ng TAMRA-Ubiquitin is sufficient for in-gel visualization of TAMRA fluorescence.
Molecular Weight:
8.9 kDa
Buffer:
20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Source:
chemical synthesis
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