Ub75 lacks the amino acid glycine 76, which is required to form thioester bond with Ub activating enzyme E1. Therefore, Ub75 can not react with E1, E2 and E3 enzymes, and does not support ubiquitination. It can be used as a negative control in ubiquitination reactions, or to assay binding of Ub with Ub binding proteins or ubiquitination enzymes. This product can be used in pulldown assays to determine ubiquitin-binding proteins.
Molecular Weight:
8.5 kDa
Buffer:
20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Source:
E. coli recombinant
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