USP30(64-502) contains the catalytic domain of USP30 with additional loops being deleted. This variant is stable and highly active compared to the commonly used USP30(57-517) (ref. 4). USP30 deubiquitinates substrates of Parkin including MIRO1, TOMM20, MFN1, and MFN2 on mitochondrial membranes and functions to suppress mitophagy. It prefers cleavage of K6- and K11-linked polyubiquitin chains.
