K63 polyUb Chain-binding Protein Identification / Validation Kit
Biozol Catalog Number:
UBP-J2510
Supplier Catalog Number:
J2510
Alternative Catalog Number:
UBP-J2510-1
Manufacturer:
UBPBio, LLC
Category:
Kits/Assays
Species Reactivity:
Human
This kit is designed for two purposes: 1) identification of proteins that specially bind K63 polyUb chains in whole cell or tissue lysates, 2) validation of direct interactions between K63 polyUb chains and their binding proteins. The non-cleavable 6xHis-tagged K63 polyUb chains (2-4) are resistant of deubiquitination when incubating with whole cell or tissue lysates, thus allowing maximally capture proteins that specifically bind K63 polyUb chains. After binding, the non-cleavable K63 polyUb chains and the binding proteins can be enriched by Nickel XPure Agarose Resin (included). The bound proteins and the non-cleavable K63 polyUb chains can be eluted from Ni resin using a buffer containing 250 mM imidazole (included) or by incubating with thrombin (not included). Either of the elution method can significantly reduce the amounts of non-specific binding proteins when compared with elution using SDS sample buffer.
Buffer:
20 mM Tris, 150 mM NaCl, 2 mM BME, 10% Glycerol
Source:
E. coli recombinant
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