RELAYTR Ubiquitination Buffer (10X)

Catalog Number: UBP-T1001-25
Article Name: RELAYTR Ubiquitination Buffer (10X)
Biozol Catalog Number: UBP-T1001-25
Supplier Catalog Number: T1001-25
Alternative Catalog Number: UBP-T1001-25-25
Manufacturer: UBPBio, LLC
Category: Sonstiges
This buffer is optimized to keep UbE1, E2 ubiquitin-conjugating enzymes and E3 ubiquitin ligases stable under room termperature for up to 4 hours. MgCl2 is included in the buffer. Addition of ATP (Catalog T1501) is required for activation of UbE1 to initiate a ubiquitination reaction. Reactions with all components but ATP can serve as the negative reaction (background).