A single terbium chelate is covalently labeled on recombinant human ubiquitin (Ub). All lysines, the N-terminal methionine and the C-terminal glycine of Ub are available for ubiquitination. Paring with Fluorescein-Ub (Catalog T2201), this product has been validated in RELAYTR FRET assays to assess formation of K48 or K63 polyubiquitin chains and autoubiquitination of E3 Ub ligases, in which excellent signal-to-background ratios were achieved.
Molecular Weight:
9.5 kda
Buffer:
20mM HEPES, pH7.5, 50 mM NaCl, 2 mM TCEP
Source:
E. Coli Recombinant
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