Time resolved (TR)-FRET offers a sensitive, low background, stable and homogenous assay for determination of protein ubiquitination. Europium-ubiquitin and Cy5-ubiquitin are a pair of modified ubiquitin suitable for TR-FRET assays to assess formation of polyubiquitin chains, thereby reporting the activity of E2 and E3 enzymes in the reaction. The concentration and molar ratio of europium-ubiquitin and Cy5-ubiquitin in the RELAYTR Europium/Cy5 Ubiquitin Mix are optimized to achieve excellent signal-to-background ratio in ubiquitination reactions. Optimization of protein concentrations is often necessary to achieve a desirable signal-to-background ratio. Typical protein concentration ranges are: UbE1 (10-30 nM), E2 (25-250 nM) and E3 (10-250 nM).
Molecular Weight:
9.5 kDa/9.1 kDa
Buffer:
See Datasheet
Source:
E. Coli Recombinant
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