RELAYTR K48 Linkage-specific Deubiquitinase Assay Kit I is based on the TR-FRET technology that offers a simple, sensitive, homogeneous, and stable assay to measure the activity of deubiquitinases (DUBs) that cleave K48-linked polyubiquitin chains. Each ubiquitin moiety in K48-linked diubiquitin (diUb) contains an HA tag or a DYKDDDDK tag, generating a robust FRET signal when binding with fluorescein-labeled anti-HA and terbium-labeled anti-DYKDDDDK antibodies. The mix of each individual ubiquitin is included as a negative control. The assay is high throughput compatible with a Z value > 0.87.
Molecular Weight:
See Datasheet
Buffer:
See Datasheet
Source:
E. Coli Recombinant
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