Fluorescein is covalently conjugated on lysines of recombinant protein G. The molar ratio between fluorescein and protein G in different lots is ~1 - 2. Protein G has higher affinity than protein A in binding of mouse, human, rat, goat and cow IgG. Fluorescein-protein G recognizes various antibodies and can serve as the acceptor in TR-FRET assays that offer a sensitive tool in monitoring molecular proximity, post-translational modifications, enzyme activity and protein abundance. The donor is usually a terbium-based chelator.
Molecular Weight:
22kDa
Buffer:
10 mM PBS (pH 7.2), 10% glycerol
Source:
Recombinant
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