Human CD137 (4-1BB) is expressed on activated T cells within 24-48 hours of activation (3). CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily (1,2). CD137 appears to be important for T cell proliferation and survival (4). Functional Application: CD137 Ig fusion protein blocks binding of anti-human CD137 to activated CEM human tumor cells and also binds to CD137 Ligand on Raji cells. Human CD137(4-1BB) huIg Fusion Protein* Molecular Structure: A soluble 55 kd fusion protein consisting of the extracellular (186aa) domain of human CD137 (4-1BB) fused to human IgG1 Fc (4). Transfectant Cell Line: CHO Applications: Suitable for use in ELISA and Flow cytometry. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
44.1
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl.
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