4E Binding Protein 1, phosphorylated (Thr37/46) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP), Clone: [9B142236B4], Rabbit, Monoclonal
Biozol Catalog Number:
USB-0004-18J
Supplier Catalog Number:
0004-18J
Alternative Catalog Number:
USB-0004-18J-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
FC, IF, IHC, WB
Immunogen:
Synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.
When bound to eIF4E, 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation. Upon hyperphosphorylation of 4E-BP1 this interaction is disrupted and cap-dependent translation is activated. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo, while phosphorylation by FRAP/mTOR on Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000. For Western Blots, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:1800-1:3200 Immunofluorescence (IC): 1:200-1:800 Flow Cytometry (Fixed/Permeabilized): 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
