4E Binding Protein 1, phosphorylated (Thr70) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP), Rabbit
Biozol Catalog Number:
USB-0004-20A
Supplier Catalog Number:
0004-20A
Alternative Catalog Number:
USB-0004-20A-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
IP, WB
Immunogen:
Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues, phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70. Applications: Suitable for use in Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blotting: 1:1000 (Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking.) Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Molecular Weight: 15-20kD Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
