14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor prot

Product Overview

• Article Name: 14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor prot
• Biozol Catalog Number: USB-0014-02
• Supplier Catalog Number: 0014-02
• Alternative Catalog Number: USB-0014-02-200
• Manufacturer: US Biological
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, IF, WB
• Immunogen: Synthetic peptide corresponding to 20aa from the N-terminus of the human 14-3-3 beta/alpha protein. Sequence Homology: delta and zeta isoforms: single aa change, tau isoform: three aa change, gamma isoform: four aa change.

Product Description

14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins. 14-3-3 proteins bind to and inuence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr. Interactions between 14-3-3 and target proteins are strongly inuenced by the phosphorylation state of 14-3-3 and the target protein. 14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function. Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity. Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1.0ug/ml Western Blot: 2ug/ml Immunofluorescence (IC): 2ug/ml See reference 1 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Purity: Purified by immunoaffinity chromatography.
• Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.