The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control. Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions. Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins. Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25c, FKHRL1, PKC and c-Raf. This antibody provides a powerful tool for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughout drug discovery. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA (Peptide): 1:500 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1xTBS, 0.1% Tween-20 at 4C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:100. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form:
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
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