Biotin-4-Fluorescein is used to quantitate biotin binding sites. Biotin-4-Fluorescein is a reagent that binds to avidin more readily than conventional fluorescein biotin. This bifunctional biotin-fluorescein conjugate demonstrates better binding and stronger fluorescence than biotin fluorescein. It has similar avidin-binding properties in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. These exceptional properties are attributed to the small size/length of the new ligand since all larger/longer biotin derivatives are known for their mutual steric hindrance and anti-cooperative binding in 4:1 complexes with avidin and streptavidin tetramers. Specific binding of this biotin-fluorescein conjugate towards avidin and streptavidin is accompanied by 84-88% quenching of ligand fluorescence. It is used for the quantitation of biotin-binding sites. Both the fluorescence and absorbance of biotin-4-fluorescein are quenched upon binding to one of the four biotin-binding sites of streptavidin, avidin, or the streptavidin conjugates of fluorescent dyes or enzymes. As a result, when a known concentration of biotin-4-fluorescein is added to a known amount of streptavidin, one can estimate the number of biotin-binding sites. Synonyms: (3aS,4S,6aR)-N-[2-[[(3,6-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9-[9H]xanthen]-5-yl)carbonyl]amino]ethyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide CAS Number: 1032732-74-3 Molecular Formula: C33H32N4O8S Molecular Weight: 644.69 Purity: 98% Appearance: Light yellow to dark yellow or light orange to dark orange powder Solubility: DMSO Storage and Stability: Store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molecular Weight:
644.69
Purity:
98%
Form:
Light yellow to dark yellow or light orange to dark orange powder
CAS Number:
[1032732-74-3]
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