The Fluorescein Arsenical Hairpin Binder (FlAsH) method is presented for fluorescence labeling of proteins in living cells. Labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin binder-ethanedithiol (FlAsH-EDT2). The membrane-permeant fluorogenic biarsenicals FlAsH-EDT2 and ReAsH-EDT2 can be prepared in good yields by a straightforward two-step procedure from the inexpensive precursor dyes fluorescein and resorufin. Synonyms: 4,5-Bis(1,3,2-dithiarsolan-2-yl)-3,6-dihydroxyspiro[isobenzofuran-1(3H),9-[9H]xanthen]-3-one, FlAsH, Fluorescein Arsenical Helix Binder, Lumio Green CAS No: 212118-77-9 Molecular Formula: C24H18As2O5S4 Molecular Weight: 664.5 Purity: 96% Appearance: White to pale orange solid Melting Point: >173C (dec) Solubility: Chloroform (Slightly, Heated), Methanol (Very Slightly, Heated) Method for Determining Identity: Proton NMR (CDCl3) Spectroscopic and Mass Spectrometric Analysis TLC Conditions: SiO2, Ethyl Acetate:Hexanes = 1:1, Visualized with UV and AMCS, SIngle Spot, Rf=0.50 Elemental Analysis: Calculated: %C: 43.38, %H: 2.73 Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Light and temperature sensitive. Store under inert atmosphere.
Molecular Weight:
664.5
Purity:
96%
Form:
White to pale orange solid
CAS Number:
[212118-77-9]
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