5-Hydroxytryptamine (5-HT) BioAssay(TM) ELISA Kit (w/o Stop Solution)

Product Overview

• Article Name: 5-Hydroxytryptamine (5-HT) BioAssay(TM) ELISA Kit (w/o Stop Solution)
• Biozol Catalog Number: USB-023037-Z
• Supplier Catalog Number: 023037-Z
• Alternative Catalog Number: USB-023037-Z-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The 5-Hydroxytryptamine (5-HT) BioAssay(TM) ELISA Kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of 5-HT in serum and plasma. Detection Range: 11.11-900ng/ml Sensitivity: 3.97ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *023037A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *023037B: Standard, 2x1 vial 023037C: Standard Diluent, 1x20ml *023037D: Detection Reagent A (green), 1x1vial *023037E: Detection Reagent B (red), 1x120ul 023037F: Assay Diluent A, 1x12ml 023037G: Assay Diluent B, 1x12ml 023037H: TMB Substrate, 1x9ml *023037J: Positive Control, 1x1vial, xxng/ml (Lyophilized) 023037L: Wash Buffer, 30X, 1x20ml 023037M: Reagent Diluent, 1x300ul Storage and Stability: Store *023037A, *023037B, *023037D, *023037E and *023037J at -20C. After reconstitution of *023037J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 50ul standard or sample to each well. 3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.