Acrosin (ACR) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Acrosin (ACR) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-023089
• Supplier Catalog Number: 023089
• Alternative Catalog Number: USB-023089-48,USB-023089-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Acrosin (ACR) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of ACR in human sperm. Background: ACR is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. Detection Range: 0.156-10ng/ml Sensitivity: 0.062ng/ml Kit Components: 023089A: Microtiter Plate, 1x96 wells 023089B: Standard (lyophilized), 2x1vial 023089C: Detection Reagent A (100X), 1x120ul 023089D: Detection Reagent B (100X), 1x120ul 023089E: TMB Substrate, 1x9ml 023089F: Wash Buffer (30X), 1x20ml 023089G: Standard Diluent, 1x20ml 023089H: Assay Diluent A (2X), 1x6ml 023089J: Assay Diluent B (2X), 1x6ml 023089K: Stop Solution, 1x6ml Storage and Stability: Store 023089A, 023089B, 023089C, 023089D at -20C upon receipt. Store other components at 4C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.