Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-023137
• Supplier Catalog Number: 023137
• Alternative Catalog Number: USB-023137-48,USB-023137-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) BioAssay(TM) ELISA Kit (Human) is a competitive inhibition enzyme immunoassay for in vitro quantitative measurement of ADCYAP1 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 6.17-500pg/ml Sensitivity: 2.62pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *023137A: Microtiter Strips, 1x96 wells *023137B: Standard, 2x1 vial 023137C: Standard Diluent, 1x20ml *023137D: Detection Reagent A, 1x120ul *023137E: Detection Reagent B, 1x120ul 023137F: Assay Diluent A, 1x12ml 023137G: Assay Diluent B, 1x12ml 023137H: TMB Substrate, 1x9ml 023137K: Stop Solution, 1x6ml 023137L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (023137K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023137A, *023137B, *023137D and *023137E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450nm immediately.