| The Angiopoietin Like Protein 4 (ANGPTL4) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ANGPTL4 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.055ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *023385A: Microtiter Strips, 1x96 wells *023385B:. Standard, 2x1 vial 023385C: Standard Diluent, 1x20ml *023385D: Detection Reagent A (green), 1x120ul *023385E: Detection Reagent B (red), 1x120ul 023385F: Assay Diluent A, 1x12ml 023385G: Assay Diluent B, 1x12ml 023385H: TMB Substrate, 1x9ml *023385J: Positive Control, 2.32ng/ml, 1x1vial (Lyophilized) 023385K: Stop Solution, 1x6ml 023385L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (023385K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023385A, *023385B, *023385D, *023385E and *023385J at -20C. After reconstitution of *J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 023385J - Positive Control: Intended Use: The control vials are from a mouse serum sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contain mouse ANGPTL4 in mouse serum (lyophilized powder). Note: The mouse based serum used in this product are from healthy mouse of SPF standard (specific pathogen-free animals). Expected Value: 2.32ng/ml Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and samples. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately. |
