Annexin A1 (ANXA1) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Annexin A1 (ANXA1) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-023428-SP
• Supplier Catalog Number: 023428-SP
• Alternative Catalog Number: USB-023428-SP-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Annexin A1 (ANXA1) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ANXA1 in human serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.312-20ng/ml Sensitivity: 0.115ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *023428A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use *023428B: Standard, 2x1vial 023428C: Standard Diluent, 1x10ml or 1x20ml *023428D: Detection Reagent A (green), 1x60ul or 1x120ul *023428E: Detection Reagent B (red), 1x60ul or 1x120ul 023428F: Assay Diluent A, 1x6ml or 1x12ml 023428G: Assay Diluent B, 1x6ml or 1x12ml 023428H: TMB Substrate, 1x4.5ml or 1x9ml 023428J: Positive Control 2.11ng/ml, 1x1vial (Lyophilized) 023428K: Stop Solution, 1x3ml or 1x6ml 023428L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution: The Stop Solution (023428K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *023428A, *023428B, *023428D, *023428E and *023428J at -20C. After reconstitution of *023428-SP-J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once opened, it is highly recommended to use remaining reagents within 1 month. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.