Collagen Type I (COL1) BioAssay(TM) ELISA Kit (Rat)

Product Overview

• Article Name: Collagen Type I (COL1) BioAssay(TM) ELISA Kit (Rat)
• Biozol Catalog Number: USB-024261
• Supplier Catalog Number: 024261
• Alternative Catalog Number: USB-024261-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Collagen Type I (COL1) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL1 in rat serum, plasma and other biological fluids. Detection Range: 0.625-40ng/ml Sensitivity: 0.224ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *024261A: Microtiter Strips, 1x48 wells or1x96 wells *024261B: Standard, 2x1vial 024261C: Standard Diluent, 1x10ml or 1x20ml *024261D: Detection Reagent A (green), 1x60ul or 1x120ul *024261E: Detection Reagent B (red), 1x60ul or 1x120ul 024261F: Assay Diluent A, 1x6ml or 1x12ml 024261G: Assay Diluent B, 1x6ml or 1x12ml 024261H: TMB Substrate, 1x14.5ml or 1x9ml *024261J: Positive Control, 24.6ng/ml, 1x1vial (Lyophilized) 024261K: Stop Solution, 1x3ml or 1x6ml 024261L: Wash Buffer, 30X, 1x10ml or 1x20ml Precaution: The Stop Solution (024261K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *024261A, *024261B, *024261D, *024261E and *024261J at -20C. After reconstitution of *024261J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 024261J: Positive Control: Intended Use: The control vial is from a rat serum sample known to contain COL1. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The control vial contains rat COL1 in rat serum (lyophilized powder). Note: The rat source material used in this product is from healthy rat of SPF standards (specific pathogen-free animals). Expected Values: 24.6ng/ml Usage: Dissolve with 150ul standard diluent and let it stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.