Glutamate Cysteine Ligase, Modifier Subunit (GCLM) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of GCLM in human tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.061ng/ml Intra-Assay CV: < 10% Inter-Assay CV: < 12% Kit Components: *025331A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *025331B: Standard, 2x1 vial 025331C: Standard Diluent, 1x20ml *025331D: Detection Reagent A, 1x120ul *025331E: Detection Reagent B, 1x120ul 025331F: Assay Diluent A, 2X, 1x6ml 025331G: Assay Diluent B, 2X, 1x6ml 025331H: TMB Substrate, 1x9ml 025331K: Stop Solution, 1x6ml 025331L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (025331K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *025331A, *025331B, *025331D and *025331E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
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