Growth Regulated Oncogene Beta (GROb) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Growth Regulated Oncogene Beta (GROb) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-025516
• Supplier Catalog Number: 025516
• Alternative Catalog Number: USB-025516-48,USB-025516-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Growth Regulated Oncogene Beta (GROb) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of GROb in human serum, plasma and other biological fluids. Detection Range: 15.6-1,000pg/ml Sensitivity: 6.1pg/ml Intra-Assay: CV10% Inter-Assay: CV12% Kit Components: *025516A: Microtiter Plate, 1x96wells, Pre-coated, ready to use *025516B: Standard, 2x1vial 025516C: Standard Diluent, 1x20ml *025516D: Detection Reagent A, 1x120ul *025516E: Detection Reagent B, 1x120ul 025516F: Assay Diluent A, 1x12ml 025516G: Assay Diluent B, 1x12ml 025516H: TMB Substrate, 1x9ml 025516K: Stop Solution, 1x6ml 025516L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (025516K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *025516A, *025516B, *025516D and *025516E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul 025516H: TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul 025516K: Stop Solution. Read at 450nm immediately.