Hepatocyte Growth Factor (HGF) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Hepatocyte Growth Factor (HGF) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-025636
• Supplier Catalog Number: 025636
• Alternative Catalog Number: USB-025636-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Hepatocyte Growth Factor (HGF) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of HGF in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 125-8000pg/ml Sensitivity: 46pg/ml Intra-Assay CV: < 10% Inter-Assay CV: < 12% Kit Components: *025636A: Microtiter Strip, 1x96 wells, Pre-coated, ready to use *025636B: Standard, 2x1 vial 025636C: Standard Diluent, 1x20ml *025636D: Detection Reagent A, 1x120ul *025636E: Detection Reagent B, 1x120ul 025636F: Assay Diluent A, 1x12ml 025636G: Assay Diluent B, 1x12ml 025636H: TMB Substrate, 1x9ml *025636J: Positive Control, 1824.3pg/ml, 1x1 vial 025636K: Stop Solution, 1x6ml 025636L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (025636K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *025636A, *025636B, *025636D, *025636E and *025636J at -20C. After reconstitution of *025636J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.