Nephrin (NPHN) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Nephrin (NPHN) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-027032
• Supplier Catalog Number: 027032
• Alternative Catalog Number: USB-027032-48,USB-027032-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Nephrin (NPHN) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for in vitro quantitative measurement of NPHN in human tissue homogenates, urine and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.054ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027032A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027032B: Standard, 2x1 vial 027032C: Standard Diluent, 1x20ml *027032D: Detection Reagent A, 1x120ul *027032E: Detection Reagent B, 1x120ul 027032F: Assay Diluent A, 1x12ml 027032G: Assay Diluent B, 1x12ml 027032H: TMB Substrate, 1x9ml 027032K: Stop Solution, 1x6ml 027032L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (027032K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027032A, *027032B, *027032D and *027032E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.