Neurogranin (NRGN) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Neurogranin (NRGN) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-027086
• Supplier Catalog Number: 027086
• Alternative Catalog Number: USB-027086-48,USB-027086-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Neurogranin (NRGN) BioAssay(TM) ELISA Kit (Human) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of human NRGN in tissue homogenates, cell lysates and other biological fluids. Detection Range: 123.5-10,000pg/ml Sensitivity: 49.3pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027086A: Microtiter Strips, 1x96 wells *027086B: Standard, 2x1 vial 027086C: Standard Diluent, 1x20ml *027086D: Detection Reagent A, 1x120ul *027086E: Detection Reagent B, 1x120ul 027086F: Assay Diluent A, 1x12ml 027086G: Assay Diluent B, 1x12ml 027086H: TMB Substrate, 1x9ml *027086J: Positive Control, 3908.2pg/ml, 1x1vial 027086K: Stop Solution, 1x6ml 027086L: Wash Buffer, 30X, 1x20ml 027086M: Reagent Diluent., 1x300ul Precaution: The Stop Solution (027086K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027086A, *027086B, *027086D and *027086E and *027086J at -20C. After reconstitution of *027086J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well. And then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450nm immediately.