Phosphatase And Tensin Homolog (PTEN) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Phosphatase And Tensin Homolog (PTEN) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-027396
• Supplier Catalog Number: 027396
• Alternative Catalog Number: USB-027396-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Phosphatase And Tensin Homolog (PTEN) BioAssay(TM) ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PTEN in serum, plasma, human tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 0.312-20ng/ml Sensitivity: 0.119ng/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027396A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027396B: Standard, 2x1vial 027396C: Standard Diluent, 1x20ml *027396D: Detection Reagent A (green), 1x120ul *027396E: Detection Reagent B (red), 1x120ul 027396F: Assay Diluent A, 1x12ml 027396G: Assay Diluent B, 1x12ml 027396H: TMB Substrate, 1x9ml *027396J: Positive Control, xxng/ml, 1x1vial (Lyophilized) 027396K: Stop Solution, 1x6ml 027396L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (027396K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027396A, *027396B, *027396D, *027396E and *027396J at -20C. After reconstitution of *027396J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.