Tissue Plasminogen Activator (tPA) BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Tissue Plasminogen Activator (tPA) BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-027481
• Supplier Catalog Number: 027481
• Alternative Catalog Number: USB-027481-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Mouse Tissue Plasminogen Activator (tPA) ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of tPA in mouse serum, plasma and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: <0.067ng/ml Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to tPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to tPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain tPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of tPA in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *027481A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use. *027481B: Standard, 2x1vial 027481C: Standard Diluent, 1x20ml *027481D: Detection Reagent A, 1x120ul *027481E: Detection Reagent B, 1x120ul 027481F: Assay Diluent A, 1x12ml 027481G: Assay Diluent B, 1x12ml 027481H: TMB Substrate, 1x9ml 027481K: Stop Solution, 1x6ml 027481L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *027481A, *027481B, *027481D and *027481E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.