Prokineticin 2 (PK2) BioAssay(TM) ELISA Kit (Human) is a competitive inhibition enzyme immunoassay for in vitro quantitative measurement of PK2 in human serum, plasma and other biological fluids. Detection Range: 246.9-20,000pg/ml Sensitivity: 89.5pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027631A: Microtiter Strips, 1x96 wells *027631B: Standard, 2x1 vial 027631C: Standard Diluent, 1x20ml *027631D: Detection Reagent A (green), 1x120ul (green) *027631E: Detection Reagent B (red), 1x120ul 027631F: Assay Diluent A, 1x12ml 027631G: Assay Diluent B, 1x12ml 027631H: TMB Substrate, 1x9ml 027631K: Stop Solution, 1x6ml 027631L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (027631K) included for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027631A, *027631B, *027631D and *027631E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450nm immediately.
