Renin (REN) BioAssay(TM) ELISA Kit (Rat)

Product Overview

• Article Name: Renin (REN) BioAssay(TM) ELISA Kit (Rat)
• Biozol Catalog Number: USB-027850
• Supplier Catalog Number: 027850
• Alternative Catalog Number: USB-027850-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Renin (REN) BioAssay(TM) ELISA Kit (Rat) is a sandwich enzyme immunoassay for in vitro quantitative measurement of REN in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Detection Range: 15.6-1000pg/ml Sensitivity: 5.9pg/ml Intra-Assay: CV <10% Inter-Assay: CV <12% Kit Components: *027850A: Microtiter Strips, 1x96 wells, Pre-coated, ready to use *027850B: Standard, 2x1vial 027850C: Standard Diluent, 1x20ml *027850D: Detection Reagent A, 1x120ul *027850E: Detection Reagent B, 1x120ul 027850F: Assay Diluent A, 1x12ml 027850G: Assay Diluent B, 1x12ml 027850H: TMB Substrate, 1x9ml 027850K: Stop Solution, 1x6ml 027850L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (027850K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *027850A, *027850B, *027850D and *027850E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.