Secretory Leukocyte Peptidase Inhibitor (SLPI) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Secretory Leukocyte Peptidase Inhibitor (SLPI) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-027994
• Supplier Catalog Number: 027994
• Alternative Catalog Number: USB-027994-48,USB-027994-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Secretory Leukocyte Peptidase Inhibitor (SLPI) BioAssay(TM) ELISA Kit (Human) is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SLPI in human serum, plasma and other biological fluids. Detection Range: 62.5-4000pg/ml Sensitivity: 29.2pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *027994A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *027994B: Standard, 2x1vial 027994C: Standard Diluent, 1x20ml *027994D: Detection Reagent A (green), 1x120ul *027994E: Detection Reagent B (red), 1x120ul 027994F: Assay Diluent A, 1x12ml 027994G: Assay Diluent B, 1x12ml 027994H: TMB Substrate, 1x9ml 027994K: Stop Solution, 1x6ml 027994L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *027994A, *027994B, *027994D and *027994E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.