Thromboxane A2 (TXA2) BioAssay(TM) ELISA Kit (All Species)

Product Overview

• Article Name: Thromboxane A2 (TXA2) BioAssay(TM) ELISA Kit (All Species)
• Biozol Catalog Number: USB-028370
• Supplier Catalog Number: 028370
• Alternative Catalog Number: USB-028370-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Thromboxane A2 (TXA2) BioAssay(TM) ELISA Kit (All Species) is a competitive inhibition immunoassay for the in vitro quantitative measurement of TXA2 in plasma, serum and other biological fluids. Detection Range: 12.35-1,000pg/ml Sensitivity: 5.62pg/ml Precision: Intra-Assay CV: <10% Inter-Assay CV: <12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to TXA2 has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled TXA2 and unlabeled TXA2 in standards or samples for limited binding sites on the pre-coated antibody. After incubation, unbound materials are washed off. Next, avidin conjugated to horseradish peroxidase (HRP) is added to each microplate well and incubated. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of TXA2 in the sample. Kit Components: *028370: Microtiter Plate, 96 wells, Pre-coated, ready to use. *028370B: Standard, 2x1vial 028370C: Standard Diluent, 1x20ml *028370D: Detection Reagent A, 1x1vial *028370E: Detection Reagent B, 1x120ul 028370F: Assay Diluent A, 1x12ml 028370G: Assay Diluent B, 1x12ml 028370H: TMB Substrate, 1x9ml 028370K: Stop Solution, 1x6ml 028370L: Wash Buffer, 30X, 1x20ml 028370M: Reagent Diluent, 1x300ml Storage and Stability: Store *028370A, *028370B, *028370D and *028370E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Summary of the Assay: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well, then add 50ul Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37C. 7. Add 50ul Stop Solution. Read absorbance at 450nm immediately.