Thyroid Stimulating Hormone (TSH) BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Thyroid Stimulating Hormone (TSH) BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-028401
• Supplier Catalog Number: 028401
• Alternative Catalog Number: USB-028401-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Thyroid Stimulating Hormone (TSH) BioAssay(TM) ELISA Kit (Mouse) is a competitive inhibition immunoassay for the in vitro quantitative measurement of TSH in mouse serum, plasma and other biological fluids. Detection Range: 49.4-4000pg/ml Sensitivity: 17.5pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *028401A: Microtiter Plate, 96 wells, Pre-coated, ready to use *028401B: Standard, 2x1vial 028401C: Standard Diluent, 1x20ml *028401D: Detection Reagent A (green), 1x1vial *028401E: Detection Reagent B (red), 1x120ul 028401F: Assay Diluent A, 1x12ml 028401G: Assay Diluent B, 1x12ml 028401H: TMB Substrate, 1x9ml 028401K: Stop Solution, 1x6ml 028401L: Wash Buffer, 30X, 1x20ml 028401M: Detection Reagent A Diluent, 1x300ul Precaution: The Stop Solution (028401K) suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *028401A, *028401B, *028401D and *028401E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards, 2. Add 50ul standard or sample to each well. 3. Add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately.