Thyrotropin Releasing Hormone (TRH) BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Thyrotropin Releasing Hormone (TRH) BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-028407
• Supplier Catalog Number: 028407
• Alternative Catalog Number: USB-028407-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit is a competitive inhibition immunoassay for in vitro quantitative measurement of mouse TRH in serum, plasma other biological fluids. Detection Range: 6.17-500pg/ml Sensitivity: 2.77pg/ml Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for TRH has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled TRH and unlabeled TRH (standards or samples) with the pre-coated antibody specific for TRH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of TRH in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of TRH in the sample. Kit Components: *028407A: Microtiter Plate, 96-well strip plate. Pre-coated, ready to use *028407B: Standard, 2x1vial 028407C: Standard Diluent, 1x20ml *028407D: Detection Reagent A (green), 1x120ul *028407E: Detection Reagent B (red), 1x120ul 028407F: Assay Diluent A (2x), 1x6ml 028407G: Assay Diluent B (2x), 1x6ml 028407H: TMB Substrate, 1x9ml 028407K: Stop Solution, 1x6ml 028407L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *028407A, *028407B, *028407D and *028407E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 50ul standard or sample to each well. and hen add 50ul prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37C. 3. Aspirate and wash 3 times. 4. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 5. Aspirate and wash 5 times. 6. Add 90ul Substrate Solution. Incubate 15-25 minutes at 37C. 7. Add 50ul Stop Solution. Read at 450 nm immediately.