Troponin I Type 3, Cardiac (TNNI3) BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Troponin I Type 3, Cardiac (TNNI3) BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-028630
• Supplier Catalog Number: 028630
• Alternative Catalog Number: USB-028630-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Troponin I Type 3, Cardiac (TNNI3) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TNNI3 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. Detection Range: 31.2-2,000pg/ml Sensitivity: 11.4pg/ml Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *028630A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *028630B: Standard, 2x1vial 028630C: Standard Diluent, 1x20ml *028630D: Detection Reagent A, 1x120ul *028630E: Detection Reagent B, 1x120ul 028630F: Assay Diluent A, 1x12ml 028630G: Assay Diluent B, 1x12ml 028630H: TMB Substrate, 1x9ml *028630J: Positive Control, 1x1vial, 346pg/ml (Lyophilized) 028630K: Stop Solution, 1x6ml 028630L: Wash Buffer, 30x, 1x20ml Precaution: The Stop Solution (028630K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *028630A, *028630B, *028630D, *028630E and *028630J at -20C. After reconstitution of *028630J, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Notes on 028630J: Positive Control Intended Use: The positive control is from a mouse heart tissue homogenates sample known to contain the target protein. It will provide a positive result in the ELISA assay, which indicates that the kit is optimized and working properly. Characteristics: The positive control vial contains mouse TNNI3 in mouse heart tissue homogenates (lyophilized powder). Note: The mouse based serum used in this product are from healthy mouse of SPF standard (Specific Pathogen-Free animals). Expected Values: 346pg/ml Usage: Dissolve with 150ul standard diluent and let stand for 10 minutes. If powder is partly insoluble, shake gently until fully dissolved. Mix gently before use. In the experiment, add 100ul to an appropriate well of the plate and test its concentration along with standards and your specimens. Note: Upon receipt store Positive Control vial at -20C. After reconstitution, store Positive Control vial at 4C and use within 5 days. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate. Incubate 10-20 minutes at 37C. 8. Add 50ul Stop Solution. Read at 450nm immediately