The Mouse Tubulin Alpha 4A (TUBa4A) ELISA kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of TUBa4A in mouse tissue homogenates, cell lysates and other biological fluids.. Detection Range: 0.312-20ng/ml Sensitivity: <0.124ng/ml Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TUBa4A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TUBa4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TUBa4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of TUBa4A in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *028659A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use. *028659B: Standard, 2x1vial 028659C: Standard Diluent, 1x20ml *028659D: Detection Reagent A, 1x120ul *028659E: Detection Reagent B, 1x120ul 028659F: Assay Diluent A, 1x12ml 028659G: Assay Diluent B, 1x12ml 028659H: TMB Substrate, 1x9ml 028659K: Stop Solution, 1x6ml 028659L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *028659A, *028659B, *028659D and *028659E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Materials Required But Not Supplied: 1. Microplate reader with 450 10nm filter. 2. Precision single or multi-channel pipettes and disposable tips. 3. Eppendorf Tubes for diluting samples. 4. Deionized or distilled water. 5. Absorbent paper for blotting the microtiter plate. 6. Container for Wash Solution Sample Preparation and Storage: Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissues were rinsed thoroughly in ice-cold PBS (0.02M pH 7.0-7.2) to remove excess blood and weighed before homogenization. The tissues were minced to small pieces and homogenized in 5-10ml of PBS with a glass homogenizer on ice (Micro Tissue Grinders works too). The resulting suspension was sonicated with an ultrasonic cell disrupter or subjected to two freeze-thaw cycles to further break the cell membranes. After that, the homogenates were centrifuged for 5 minutes at 2500*g. The supernatant was removed and assayed immediately or aliquoted and stored at -20C or lower. Cell Lysates: Cells must be lysed before assaying according to the following directions. 1. Adherent cells should be detached with trypsin and then collected by centrifugation (suspension cells can be collected by centrifugation directly). 2. Wash cells three times in cold PBS. 3. Resuspend cells in PBS (1*) and subject the cells to ultrasonication 4X (or freeze cells at -20C. Thaw cells with gentle mixing. Repeat the freeze/thaw cycle 3X.) 4. Centrifuge at 1500*g for 10 minutes at 2-8C to remove cellular debris. Other Biological Fluids: Centrifuge samples for 20 minutes at 1000*g. Remove particulates and assay immediately or store samples in aliquots at -20C or -80C. Avoid repeated freeze/thaw cycles. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37C. 6. As
