Vasoactive Intestinal Peptide (VIP) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Vasoactive Intestinal Peptide (VIP) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-028858
• Supplier Catalog Number: 028858
• Alternative Catalog Number: USB-028858-48,USB-028858-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Human Vasoactive Intestinal Peptide (VIP) ELISA Kit is a competitive inhibition immunoassay for the in vitro quantitative measurement of VIP in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids. Detection Range: 6.17-500pg/ml Sensitivity: 2.34pg/ml Precision: Intra-Assay CV: <10% Inter-Assay CV: <12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific for VIP has been pre-coated onto a microplate. A competitive inhibition reaction occurs between biotin-labeled VIP and unlabeled VIP (standards or samples) with the pre-coated antibody specific for VIP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is inversely proportional to the concentration of VIP in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of VIP in the sample. Kit Components: *028858A: Microtiter Plate, 96 wells, Pre-coated, ready to use *028858B: Standard, 2x1vial 028858C: Standard Diluent, 1x20ml *028858D: Detection Reagent A, 1x120ul *028858E: Detection Reagent B, 1x120ul 028858F: Assay Diluent A, 1x12ml 028858G: Assay Diluent B, 1x12ml 028858H: TMB Substrate, 1x9ml 028858K: Stop Solution, 1x6ml 028858L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *028858A, *028858B, *028858D and *028858E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.