Vitamin D Receptor (VDR) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Vitamin D Receptor (VDR) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-028899
• Supplier Catalog Number: 028899
• Alternative Catalog Number: USB-028899-48,USB-028899-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Human Vitamin D Receptor (VDR) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of VDR in human tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.625-40ng/ml Sensitivity: < 0.228ng/ml Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to VDR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VDR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of VDR in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *028899A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use *028899B: Standard, 2x1vial 028899C: Standard Diluent, 1x20ml *028899D: Detection Reagent A, 1x120ul *028899E: Detection Reagent B, 1x120ul 028899F: Assay Diluent A, 1x12ml 028899G: Assay Diluent B, 1x12ml 028899H: TMB Substrate, 1x9ml 028899K: Stop Solution, 1x6ml 028899L: Wash Buffer, 30x, 1x20ml Storage and Stability: Store *028899A, *028899B, *028899D and *028899E at -20C. Store all the other components at 4C. Unused kit is stable for 6 months. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.