SUMF1, Recombinant, Human, aa91-374, His-Tag (Sulfatase-modifying Factor 1, C-alpha-formylglycine-generating Enzyme 1, FGE, PSEC0152, UNQ3037/PRO9852)

Catalog Number: USB-029703
Article Name: SUMF1, Recombinant, Human, aa91-374, His-Tag (Sulfatase-modifying Factor 1, C-alpha-formylglycine-generating Enzyme 1, FGE, PSEC0152, UNQ3037/PRO9852)
Biozol Catalog Number: USB-029703
Supplier Catalog Number: 029703
Alternative Catalog Number: USB-029703-50,USB-029703-100,USB-029703-250,USB-029703-500
Manufacturer: US Biological
Category: Molekularbiologie
SUMF1 (Sulfatase-modifying factor 1) belongs to the SUMF family. SUMF1 is an enzyme that catalyzes the hydrolysis of sulfate esters by oxidizing a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also known as C-alpha-formylglycine. Mutations in this gene cause multiple sulfatase deficiency, a lysosomal storage disorder. Recombinant protein corresponding to aa91-374 from human SUMF1, fused to His-tag at N-terminal expressed in E. coli. Accession Number: NP_877437 Molecular Weight: ~34.1kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVPIPAGVFT MGTDDPQIKQ DGEAPARRVT IDAFYMDAYE VSNTEFEKFV NSTGYLTEAE KFGDSFVFEG MLSEQVKTNI QQAVAAAPWW LPVKGANWRH PEGPDSTILH RPDHPVLHVS WNDAVAYCTW AGKRLPTEAE WEYSCRGGLH NRLFPWGNKL QPKGQHYANI WQGEFPVTNT GEDGFQGTAP VDAFPPNGYG LYNIVGNAWE WTSDWWTVHH SVEETLNPKG PPSGKDRVKK GGSYMCHRSY CYRYRCAARS QNTPDSSASN LGFRCAADRL PTMD Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 34.1
NCBI: 877437
UniProt: Q8NBK3
Purity: ~85% (SDS-PAGE, reducing conditions)
Form: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 2M urea, 20% glycerol, 2mM DTT.